Figure 6.

Expression of Rab5:S34N mutant hinders the internalization of GLP-1 receptor following orthosteric activation and blunts the receptor mediated cyclic AMP generation. A. BRIN-BD11 pancreatic beta cells were transfected with or without Rab5:S34N mutant and 48 h post transfection assessed for GLP-1Tmr internalization at 0 min and 30 min. The data are representative of three independent experiments (n = no. of cells assessed for each time point n = 23). B. BRIN-BD11 pancreatic beta cells were transfected with Rab5:S34N mutant and, 24 h post transfection, assayed for GLP-1R mediated cyclic AMP generation as determined by Cre-Luciferase assay. The data are presented as a 4-parameter logistic curve analyzed in Graph pad prism (version 6.0), and each data point is assessed in duplicates Dose response curve represents mean ± SEM of 3 independent experiments.