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. 2017 Aug 5;6(10):1198–1211. doi: 10.1016/j.molmet.2017.07.015

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Figure S6 — Additional analysis of the differentially expressed genes in the FACS-sequencing data. (A) Floating mature brown adipocytes from the female virgin transgenic mice were collected for following FACS experiment. (B) Cell suspension of mature brown adipocytes was stained for DAPI to remove the dead cells, and the mature brown adipocytes were gated by the cell size and GFP. (C) Flow cytometry analysis of mammary cells using KRT14 antibody in the red open histogram compared to concentration matched negative FMO control in blue histogram. The distribution of KRT14 in Lin⁻ population consist between virgin and lactation state. (D) Flow cytometry analysis of mammary cells using KRT8 antibody in the red open histogram compared to concentration matched negative FMO control in blue histogram. The distribution of KRT8 in Lin⁻ population difference between virgin and lactation state.