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. 2017 Aug 5;6(10):1198–1211. doi: 10.1016/j.molmet.2017.07.015

Figure 1.

Figure 1

Myf5-GFP cells could convert into mammary basal myoepithelial cells during the reproductive stage. (A) The UCP1 expression level in BAT during lactation (middle) compared to the virgin state (left). The percentage of UCP1 expression in Myf5-GFP cells shows in histogram (right). (B and C) In the virgin stage of the Myf5-Cre-ROSAmTmG mice, the interscapular BAT was stained for mammary markers KRT14 and KRT8. (D and E) During lactation, Myf5-GFP cells in the scapular region expressed the mammary basal myoepithelial markers KRT14 and KRT5. (F and G) Myf5-GFP cells were negative for the mammary luminal markers KRT8 and CSN2. (H) Flow cytometry analysis of CD24 and CD29 of Lin− cells showing Myf5-GFP expression in the anterior dorsal interscapular region mammary basal cell populations. (I and J) The Myf5-GFP cells percentage in KRT14+ mammary basal myoepithelial population (defined as Lin−:CD24+:CD29hi:Krt14+) during lactation (I) and virgin stage (n = 3) (J). (K) The Krt14+ mammary basal myoepithelial population were sorted out to observe Myf5-GFP cells. Scale bar: 20 μm. The data represent means + s.e.m. *P <0.05.