Figure 6.

The pH dependence is shown. A, the distribution of single-molecule n-F₀F₁ power stroke data sets as a function of the percentage of the occurrence of transient dwells per data set, which were binned to each 10% (gray bar graphs). Each data set contained ∼300 power strokes. The fit of the data to the sum of three Gaussians (black line) where the probability of forming transient dwells was low (blue line), medium (red line), and high (green line). The total number of n-F₀F₁ molecules examined at each pH is indicated. B, the distribution of the extent of F₀-dependent CW rotation against the force of F₁-ATPase–driven rotation. C, the difference in extent of CW rotation at each pH versus that observed at pH 5.5.