Figure 6.

Transcription start site and sequences of the plpA-regulatory region. A, the transcription start site of plpA was determined by primer extension of the RNA isolated from the wild type grown to an A₆₀₀ of 0.9. Lanes C, T, A, and G, nucleotide sequencing ladders of pKK1505. The asterisk indicates the transcription start site of plpA. B, the transcription start site of plpA is indicated by a bent arrow, and the positions of the putative −10 and −35 regions are underlined. The sequences for binding of HlyU (HLYUB1, HLYUB2, and HLYUB3; gray boxes) and CRP (CRPB; white box) were determined later in this study (Fig. 8, C and D). The consensus sequences for binding of CRP (32) are indicated above the V. vulnificus DNA sequence. x, any nucleotide.