Figure 3.

Knockdown of Klf4 abrogates the self-renewal-promoting effect of Gbx2 in mESCs. A and B, Western blot or qRT-PCR analysis of Klf4 and Gbx2 expression in PB-Gbx2 46C mESCs infected with Klf4 shRNA lentivirus. The data are presented as the means ± S.D. of three independent experiments. **, p < 0.01 versus scramble. C and D, AP staining of colonies arising from Klf4 knockdown mESCs carrying PB-Gbx2 transgenes cultured in serum medium without LIF for 7 days. Bar, 100 μm. E, quantification of AP positive colonies in Fig. 3D. The data represent means ± S.D. from triplicate experiments. F, immunofluorescence staining of NANOG in Klf4 knockdown 46C mESCs overexpressing PB-Gbx2. Bar, 100 μm.