Figure 4.

Schematic illustration of reporter constructs utilized in split firefly luciferase assay to detect HuR dimerization. A, the HuR constructs to detect HuR dimerization. Nluc corresponds to amino acids 1–398 of firefly luciferase, and Cluc corresponds to amino acids 394–550 of firefly luciferase. Both were fused with HuR (see “Experimental procedures” for details). B, sets of control constructs utilized in the split firefly luciferase assay (see “Experimental procedures” for details).