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. 2017 Aug 25;292(41):16921–16932. doi: 10.1074/jbc.M117.803346

Figure 2.

Figure 2.

Topo IV stabilizes MukB on DNA. A, Topo IV-MukB-DNA complex is less salt-sensitive than the MukB-DNA complex. Standard reaction mixtures containing nicked DNA, MukB (31 nm), and Topo IV (63 nm) were incubated for 5 min at 37 °C. KCl was then added to the final concentrations indicated, and the incubation continued for 5 min. Protein-DNA complexes formed were then analyzed by gel electrophoresis as described in the legend to Fig. 1A. B, MukBDNA variant is inefficient at DNA condensation. The indicated concentrations of either wild-type or MukB R187E/R189E (MukBDNA) were incubated with nicked DNA for 5 min at 37 °C, and protein-DNA complexes formed were analyzed by electrophoresis as described in the legend to Fig. 1A. C, in the presence of Topo IV, the MukBDNA variant can condense DNA. Reaction mixtures containing nicked DNA, either in the presence (63 nm) or absence of Topo IV, and containing the indicated concentrations of the MukBDNA variant were incubated for 5 min at 37 °C, and the protein-DNA complexes formed were analyzed as described in the legend to Fig. 1A.