Figure 4.

ParC is sufficient to form maximally compacted DNA with MukB. A, reaction mixtures containing nicked DNA, MukB (31 nm), and either Topo IV (63 nm) or ParC (63 nm), as indicated, were incubated for 5 min at 37 °C, and the protein-DNA complexes formed were analyzed as described in the legend to Fig. 1A. B, lane profiles of the gel shown in A.