Figure 7.

Nucleoids in the mukBD697KD745KE753K mutant strain are decondensed. Wild-type (BW30270) and mukB^triple (PN143) E. coli cells were grown to mid-log phase in MOPS medium. Cells were harvested, and spermidine nucleoids were prepared and sedimented through sucrose gradients as described under “Experimental procedures.” This figure is identical to Fig. 8 of the accompanying article (45) except that the gradient for PN141 has been removed from this image. A, photograph of typical sucrose gradients showing the positions of the sedimented nucleoids (white arrows). B, densitometric trace of A₂₆₀ absorbing material in each gradient.