Figure 12.

mukBK761E/R765E mutant cells are longer, and their nucleoids are larger than wild type. A, fields of DAPI-stained wild-type (BW30270) and mutant PN141 (BW30270mukBK761E/R765E) E. coli cells. B, the mutant strain is somewhat longer than the wild type. Cell length distribution of BW30270 and PN141 grown to early log phase in LB medium. A total of 1641 and 1717 cells of the wild type and PN141, respectively, were measured from three independent experiments. C, mutant strain has a somewhat larger average nucleoid area than the wild type. A total of 1630 and 1610 nucleoids in wild-type and PN141 cells, respectively, was measured from three independent experiments. The very diffuse nucleoids present in the filamented cells of PN141 were excluded from the latter measurement.