Figure 7.

MukB K761E/R765E variant protein can form both the FMcx and SMcx. A, schematic of the structure of the hinge domain of MukB (23) with the Lys-761 and Arg-765 amino acids shown in stick representation. B, MukB KE,RE can form both the FMcx and SMcx protein-DNA complexes. Either wild type or MukB KE,RE was incubated with the nicked DNA for 5 min at 37 °C, and the products were analyzed by electrophoresis through agarose gels that either did or did not contain 20 μg/ml chloroquine in the gel and running buffer. C, wild type and MukB KE,RE binding to a 5′-end-labeled 50-bp duplex DNA is identical. Nitrocellulose filter-binding assays were performed as described under “Experimental procedures.”