Table 3.
Antibodies used in this study
| Antibody | Incubation buffer | Dilution | Source |
|---|---|---|---|
| Anti-αKGD | Milk 5% | 1:200 | Dr. A. Tzagoloff (Columbia University)a |
| Anti-cytochrome b2 | Milk 5% | 1:200 | Dr. A. Tzagoloff (Columbia University)a |
| Anti-ScoI | Milk 5% | 1:200 | Dr. A. Tzagoloff (Columbia University)a |
| Anti-Prx1 | Milk 5% | 1:2000 | This studyb |
| Anti-Trx3 | Milk 5% | 1:1000 | This studyb |
| Anti-Trr2 | Milk 5% | 1:1000 | This studyb |
| Anti-HA | Milk 5% | 1:2000 | Sigma (H6908) |
| Anti-Prx3 | TBST | 1:4000 | AbFrontierLF (MA0044) |
| Anti-Porin | Milk 5% | 1:10,000 | Thermo Fisher Scientific (16G9E6BC4) |
| Anti-Pgk1 | Milk 5% | 1:10,000 | Nordic Immunology (NE130/7S) |
| Anti-Dpm1 | Milk 5% | 1:1000 | Thermo Fisher Scientific (5C5A7) |
| Anti-Vma2 | Milk 5% | 1:1000 | Thermo Fisher Scientific (11D11B2) |
| Anti-Pep12 | Milk 5% | 1:1000 | Thermo Fisher Scientific (2C3G4) |
| Anti-rabbit IgG HRP-linked | Milk 5% | 1:10,000 | Cell Signaling Technology |
| Anti-mouse IgG HRP-linked | Milk 5% | 1:10,000 | Cell Signaling Technology |
a This was produced in the laboratory of Dr. A. Tzagoloff after immunization of rabbits with recombinant proteins.
b Antibodies were produced in 2-month-old male rabbits in the Animal Care Facility of the Butantan Institute.