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. 2017 Sep 28;36(20):3046–3061. doi: 10.15252/embj.201696253

Figure EV4. Extinction of DKO ISCs is not mediated by Notch signaling downregulation or p53 activation.

Figure EV4

  1. qRT–PCR analysis of Notch, BMP, and Hedgehog target genes in sorted GFP+ cells isolated from crypts of CKIε−/−‐VillinCreER‐Lgr5CreER (CKIε−/−) and DKO‐VillinCreER‐Lgr5CreER mice (DKO) 2.5 days after KO induction. Data represent mean of three independent sorting experiments, each with a pool of six mice; t‐test was performed, ns = non‐significant. Error bars indicate SEM.
  2. qRT–PCR analysis of p53 targets in sorted GFP+ and GFP− cells isolated from crypts of CKIε−/−‐VillinCreER‐Lgr5CreER (CKIε−/−) and DKO‐VillinCreER‐Lgr5CreER (DKO), 2.5 days after KO induction. Data represent mean of three independent sorting experiments, each with a pool of six mice; t‐test was performed, *P < 0.05 **P < 0.01. Error bars indicate SEM.
  3. qRT–PCR analysis of ISC markers in IECs isolated from CKIδ hetero (hetero; n = 3), DKO (n = 3) and TKO (n = 3) mice (mean ± SEM), 5 days after KO induction; t‐test was performed, ns = non‐significant.