Figure 2. In vivo interaction between release factors and UPF proteins.

1. Co‐immunoprecipitation from RNaseA‐treated lysates of HeLa cells transfected with FLAG‐eRF1 (lanes 1–10) or unfused FLAG (lanes 11–15) and V5‐eRF3a, V5‐UPF1, V5‐UPF2, V5‐UPF3B or V5‐PYM. Co‐precipitated proteins were detected using an anti‐V5 antibody. Lysate used for the immunoprecipitations was loaded in the input lanes (left). Re‐probing with anti‐TUBB antibody served as loading control.
2. Co‐IP experiment as in (A) with FLAG‐eRF3a. Re‐probing with anti‐ACTB served as loading control.

Data information: Each panel represent two independent experiments. Because TUBB migrates at virtually the same position as FLAG‐eRF3a and ACTB migrates very closely to FLAG‐eRF1, TUBB was used as loading control for (A) and ACTB for (B). Source data are available online for this figure.