Fig. 4.

Recruitment of TAP1 and MHC class I molecules to phagosomes is VAMP8-independent. (A) Representative confocal micrographs showing the recruitment of TAP1 (green in merge) and VAMP8 (magenta) to zymosan-containing phagosomes by immunofluorescence in VAMP8 KD and NT human DCs. Arrow heads indicate phagosomes positive for TAP1. The graph shows the quantification by manual counting of TAP1-positive phagosomes of DCs from 3 individual donors. (B) Same as panel A, but now for MHC class I (MHC-I; green in merge). Scale bars, 10 μm; ns: not significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)