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. 2017 Oct 9;5(19):e13459. doi: 10.14814/phy2.13459

Figure 6.

Figure 6

Dex induced ERK1/2 phosphorylation, which was required for mRNA expression of DUSP5 and DUSP6. (A) Primary human myotubes were incubated with 0.5 μmol/L Dex for 15–240 min and phosphorylation of ERK1/2 was monitored. (B) Cells were incubated with 10 μmol/L PD for 1 h prior to incubation with Dex (15 min), and phosphorylation of ERK1/2 was monitored. (C) DUSP5 and DUSP6 (D) mRNA expression were monitored by qPCR after exposure to PD (1 h), Dex (1 h) and a combination of both, respectively. Data represent means ± SEM; P‐values were calculated by one‐way ANOVA. *P < 0.05 versus control, # P < 0.05 versus Dex, n ≥ 3. Dex, dexamethasone; ERK, extracellular signal‐regulated kinase; DUSP, dual specificity phosphatase; PD, MEK inhibitor PD98059; qPCR, quantitative real‐time PCR.