Fig. 9.

Confocal microscopy of MG-63 cells seeded on ST1 and ST2 - day 3 and day 7. Confocal microscopy of MG-63 cells seeded on ST1 (a, c, e) or ST2 (b, d, f) scaffolds from polylactic acid after a 3-day culture (a, b) or a 7-day culture (c-f). Cells were fixed and cell membranes were stained using DiOC6 (3) (green), cell nuclei were stained with propidium iodide (red). Both maximum projections (a-d) and color coded projections (e, f), which display depth (d) distribution of cells (d = 100 μm in E, d = 400 μm in F) showed fast growth of MG-63 cells on both scaffolds and formation of bridges from cells connecting fibres on ST1 scaffolds on day 7. Objective ×10, Magn. ×2, Bar = 100 μm