|
| |
| MightyAmp DNA Polymerase | 1 μl |
|
| |
| PCR mix | 25 μl |
|
| |
| Forward primer (10 μM) | 1.5 μl |
|
| |
| Reverse primer (10 μM) | 1.5 μl |
|
| |
| Bisulfite-converted DNA | 5–10 μl |
|
| |
| Total reaction volume | 50 μl |
|
| |
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