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Pharmaceutical treatments of native T cells did not affect 2D TCR–pMHC off-rate. (A) Lack of influence of perturbing cellular environment on lifetimes of OT1 TCR bonds with OVA:H2-Kbα3A2 and G4:H2-Kbα3A2 at 0 and 10 pN. Data are shown as mean + SEM of >100 lifetime events and are pooled from one to five independent experiments. (B–E) Lifetime distributions corresponding to panel (A).
