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. 2017 Oct 11;8:1275. doi: 10.3389/fimmu.2017.01275

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Copyright © 2017 Gimenez, Lima, Françoso, Denapoli, Panatieri, Bargieri, Thiberge, Andolina, Nosten, Renia, Nussenzweig, Nussenzweig, Amino, Rodrigues and Soares.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Specificity of IgG antibody in mice immunized with Plasmodium vivax circumsporozoite protein (CSP) recombinants. (A) Schematic representation of recombinant proteins used as a substrate bound to the plates in the ELISA assay. (B) C57BL/6 mice were immunized s.c. with the purified proteins in the presence of the adjuvant Poly(I:C) according to the timeline described in Figure 3A. The specificity of IgG antibody serum titers toward rFliC, FliC-PvCSP-repeats and No repeats proteins were analyzed by ELISA in sera of mice from groups 2 to 6 described in Figure 3B. The results are expressed as mean ± SD (n = 6 animals/group). P values are indicated. Differences were considered statistically significant when P < 0.05.