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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Pharmacol Res. 2017 Feb 14;119:242–250. doi: 10.1016/j.phrs.2017.02.013

Figure 2.

Figure 2

2ME treatment induces senescence and cell cycle arrest in human melanoma cells. (A) Senescence-associated β-galactosidase cytochemical detection after treatment with 1.0 μM of 2ME for 5 days. (B) Immunoblotting for Histone H3 trimethyl Lysine 9 (H3K9ME3) after treatment with 1.0 and 10.0 μM of 2ME for 5 days. β-actin was used as a loading control. (C) Cell cycle progression evaluation by flow cytometry after 2ME treatment for 24 and 48h. (D) Western Blotting analysis of cell cycle-related proteins after treatment with 0.5 and 1.0 μM of 2ME for 5 days. GAPDH was used as a loading control. Experiments were performed in triplicates.