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. 2017 Aug 1;158(11):2222–2232. doi: 10.1097/j.pain.0000000000001027

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Copyright © 2017 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The International Association for the Study of Pain.

This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — No changes in CB2 transcript levels in the periaqueductal grey (PAG) and rostroventral medulla (RVM) whilst the expression of the GPR55 gene is developmentally regulated. (A and B) TaqMan RT-PCR analysis oCB2 receptor mRNA in the PAG and RVM, respectively, no significant changes in were detected. (C and D) TaqMan RT-PCR analysis of GPR55 receptor mRNA in the PAG and the RVM of P10, P21, and P40 rats (P < 0.001; 1 way analysis of variance [ANOVA]). No changes were observed in the PAG, but GPR55 mRNA was transiently upregulated at P21 in the RVM (D). N = 3 to 5 animals per age group. Data shown here represent mean ± SEM. ** = P < 0.01, between age comparisons, 1-way ANOVA with Bonferroni multiple comparisons.