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. 2017 Aug 1;158(11):2222–2232. doi: 10.1097/j.pain.0000000000001027

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Copyright © 2017 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The International Association for the Study of Pain.

This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Changes in the expression of endocannabinoid receptors CB1 and CB2 in the human midbrain during postnatal maturation. (A and B) TaqMan real-time (RT) PCR analysis of CB1 and CB2 receptor mRNA, no significant changes in were detected. (C) In situ hybridisation images (×20 magnification) of CB1 receptors in preterm, term, infant, and P40 midbrain. (D) Quantification by cell-counting analysis showed that the expression of CB1 receptors is higher during early postnatal development compared with P40 (P < 0.01; 1-way analysis of variance [ANOVA]). (E) In situ hybridisation images of CB2 receptors. (F) No significant changes in the expression of CB2 receptors were observed throughout postnatal development. Four to 8 human midbrain tissue per age group was used for TaqMan RT-PCR and in situ hybridisation, respectively. Data shown here represent mean ± SEM. * and ** = P < 0.05 and P < 0.01, respectively, between age comparison, 1-way ANOVA with Bonferroni multiple comparisons.