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. 2017 Sep 27;51(5):1497–1507. doi: 10.3892/ijo.2017.4137

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Copyright: © Liao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Transwell invasion and migration assay and β-galactosidase staining assay between CaSki-RACK1 cells and CaSki-vector cells. (A) Transwell migration assay. (B) Transwell invasion assay. (C) Statistical analysis of cell number for Transwell invasion and migration assays. Results are presented as mean ± SD from three independent experiments. (D) mRNA expression levels of MMP-2, MMP-3, MMP-9 and MMP-10. (E and F) β-galactosidase staining and statistical analysis of cell numbers for β-galactosidase staining assay. The assay was conducted as three independent experiments. ^*P<0.05; ^**P<0.01; ^***P<0.001.