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. 2017 Sep 25;51(5):1415–1426. doi: 10.3892/ijo.2017.4135

Figure 3.

Figure 3

HCMV gene expression. (A) RT-PCR detection of IE1, US28, and β 2.7 kb transcripts in HACMV AD169-infected HT29 stem-like cells. M, 100 bp DNA ladder; P, positive control; and N, negative control. (B) Semi-quantitative SYBR Green-based RT-qPCR of IE1, US28, and β 2.7 kb at different time-points following HCMV AD169 infection of HT29 stem-like cells. ****p<0.0001 by two-way ANOVA and Tukey's multiple comparisons test. (C) Determination of HCMV AD169 long-term infection in HT29 stem-like cells (4 weeks).