Figure 4.

Cell viability and proliferation of AD169-infected and non-infected colorectal cancer-derived cells. HCMV AD169 was used to infect 10² parental and stem-like (A) HT29 cells and (B) SW480 cells at MOI of 5. Cells were harvested at 6, 12 24, 48 and 72 h following infection. Cells were subjected to WST-1 assays. AD169-infected cells showed significant proliferation compared to the non-infected cells. ^**p<0.01, ^**p<0.001 and ^***p<0.0001 by Student's t-test. For direct cell proliferation evaluation, 10⁴ parental and stem-like (C) HT29 cells and (D) SW480 cells were infected with AD169 at MOI of 5. Cells were harvested at 3, 6, 12 24, 48 and 72 h following infection and stained with 0.4% trypan blue. Cells were counted using the Countess Automated Cell Counter. ^*p<0.05, ^**p<0.01, and ^****p<0.0001 by two-way ANOVA and Tukey's multiple comparisons test.