Figure 6.

Human EMT RT² Profiler PCR Array of AD169-infected and non-infected cells. (A) HT29 stem-like cells were treated with or without HCMV AD169 at MOI of 5. After different time-points, cells were harvested and the total RNA was extracted and converted to cDNA. The hierarchical clustering of gene signatures was determined using RT² Profiler PCR Array of EMT and illustrated as heat maps (cut off value >2). (B) The expression of EMT markers and drivers gene of HT29 stem-like cells infected with AD169. (C) Expression of genes related to the WNT signaling pathway in HT29 stem-like cells infected with AD169. (D) Confirmation of WNT11/FZD7 expression in HT29 stem-like cells infected with AD169 and non-infected cells. ^*p<0.05, ^**p<0.01, and ^****p<0.0001 by two-way ANOVA and Tukey's multiple comparisons test.