Table I.
Gene | Forward (5′–3′) | Reverse (5′–3′) |
---|---|---|
RT-PCR | ||
IE1 | CGACGTTCCTGCAGACTATG | TCCTCGGTCACTTGTTCAAA |
US28 | GTGAACCGCTCATATAGACC | GAAACAGGCAGTGAGTAACG |
β 2.7 kb | AAGATGTTGCGATGCGGTTG | CGGTCAGCAGCCAAACAATC |
GAPDH | ACCACAGTCCATGCCATCAC | TCCACCACCCTGTTGCTGTA |
qPCR | ||
IE1 | AAGCGGCCTCTGATAACCAAG | GAGCAGACTCTCAGAGGATCG |
US28 | GTACCACAGCATGAGCTTTTC | GTATAATTTGTGAGACGCGACA |
β 2.7 kb | AAGATGTTGCGATGCGGTTG | CGGTCAGCAGCCAAACAATC |
Wnt 11 | GACACAAGACAGGCAGTG | GTCCTTGAGCAGAGTCCT |
FZD7 | AAGACTTGCAGGACGATGCT | TGTATCTCCCACTCGCCTTC |
CTNNB | GTGCTATCTGTCTGCTCT | CATCCCTTCCTGTTTAGTTG |
GSK3β | AAGTTAGCAGAGACAAGGA | CGCAATCGGACTATGTTAC |
GAPDH | CTGCCCCCTCTGCTGATG | TCCACGATACCAAAGTTGTCATG |