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. 2017 Sep 25;51(5):1415–1426. doi: 10.3892/ijo.2017.4135

Table I.

The primers used for RT-PCR and real-time PCR.

Gene Forward (5′–3′) Reverse (5′–3′)
RT-PCR
 IE1 CGACGTTCCTGCAGACTATG TCCTCGGTCACTTGTTCAAA
 US28 GTGAACCGCTCATATAGACC GAAACAGGCAGTGAGTAACG
 β 2.7 kb AAGATGTTGCGATGCGGTTG CGGTCAGCAGCCAAACAATC
 GAPDH ACCACAGTCCATGCCATCAC TCCACCACCCTGTTGCTGTA
qPCR
 IE1 AAGCGGCCTCTGATAACCAAG GAGCAGACTCTCAGAGGATCG
 US28 GTACCACAGCATGAGCTTTTC GTATAATTTGTGAGACGCGACA
 β 2.7 kb AAGATGTTGCGATGCGGTTG CGGTCAGCAGCCAAACAATC
 Wnt 11 GACACAAGACAGGCAGTG GTCCTTGAGCAGAGTCCT
 FZD7 AAGACTTGCAGGACGATGCT TGTATCTCCCACTCGCCTTC
 CTNNB GTGCTATCTGTCTGCTCT CATCCCTTCCTGTTTAGTTG
 GSK3β AAGTTAGCAGAGACAAGGA CGCAATCGGACTATGTTAC
 GAPDH CTGCCCCCTCTGCTGATG TCCACGATACCAAAGTTGTCATG