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. 2017 Aug 10;8(41):69237–69249. doi: 10.18632/oncotarget.20144

Figure 1. Targeting MUC1-C downregulates BMI1 expression.

Figure 1

A.-C. RPMI8226 (A), OPM-2 (B), and primary MM (C) cells from Patient #1 stably expressing a control shRNA (CshRNA) or a MUC1 shRNA were analyzed for BMI1 mRNA levels by qRT-PCR (left). The results (mean±SD of 3 determinations) are expressed as relative mRNA levels as compared with that obtained for the CshRNA cells (assigned a value of 1). Lysates were immunoblotted with the indicated antibodies (right). D. RPMI8226 cells were stably transduced to express a Tetracycline-inducible control shRNA (Tet-CshRNA) or a MUC1shRNA (Tet-MUC1shRNA). Cells treated with 200 ng/ml DOX for 5 d were analyzed for BMI1 mRNA levels by qRT-PCR (left). The results (mean±SD of 3 determinations) are expressed as relative mRNA levels compared with that obtained for control DOX-treated Tet-CshRNA cells (assigned a value of 1). Lysates were immunoblotted with the indicated antibodies (right). E. OPM-2 cells transiently transduced to express an empty vector or one expressing MUC1-C were analyzed for BMI1 mRNA levels by qRT-PCR (left). The results (mean±SD of 3 determinations) are expressed as relative mRNA levels as compared with that obtained for the vector cells (assigned a value of 1). Lysates from were immunoblotted with the indicated antibodies (right).