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. 2017 Jun 27;8(41):69577–69593. doi: 10.18632/oncotarget.18632

Figure 4. The effects of EA.hy926 conditioned medium on the invasive ability of HepG2 cells.

Figure 4

(a) Typical optical images of HepG2 cells which crossed through the pores of Transwell chamber and stained with crystal violet, illustrating invasive cells stimulated by VECM. Scale bar=30μm. The numbers of invasive cells from the images were quantitatively analyzed. *, P<0.05 denotes statistically significant difference compare with control. (b) The expressions of MMP-2 and MMP-9 in HepG2 cells. Their expression levels were quantified based on image analysis of the Western blot bands. *, P<0.05 denotes statistically significant difference compare with other groups. (c) The concentration of the MMP-2 and MMP-9 secreted by HepG2 cells with VECM. The initial concentrations of MMP-2 and MMP-9 in HCCM are defined as controls. Values represent the Mean±S.D from three independent experiments.