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. 2017 Jun 27;8(41):69622–69640. doi: 10.18632/oncotarget.18710

Figure 6. Constitutively active Akt partially rescues ESE-1 KD mediated inhibition in growth.

Figure 6

(A) BT474 cells were transfected with a Flag tagged Myr-AKT expressing plasmid or the vector control followed by transduction with the scramble control or shESE-1_1.3. (B) SKBR3 cells were transfected with a Myr Akt (untagged) plasmid or the vector control followed by transduction with the scramble control or shESE-1_1.3. In both graphs, cells labeled as Control bears the scramble control, cells labeled as Control + Myr Akt bears the scramble control and the Myr Akt plasmid. Cells labeled KD + Myr Akt bears shESE-1_1.3 and Myr Akt. Data shown are the average of three independent experiments, each of the Con + Myr Akt, and the KD, was normalized to Control. The KD+Myr Akt was normalized to Control+Myr Akt. The value of Control was set to 1. The p-value (p=0.027) for BT474 cells and p=0.022 in SKBR3 cells were determined using three replicates for each cell type by an unpaired T test in Graphpad prism. Error bars are +/- SEM derived from all three experiments.