Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 13;8(41):69731–69745. doi: 10.18632/oncotarget.19227

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © 2017 Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

(A) Effect of SB203580 on induction of Nur77 expression by CCE9. HeLa229 cells treated with 10 μM CCE9 for 0, 0.25, 0.5, 1, 3 hr in the presence or absence of 25 μM SB203580 were analyzed by Western blotting. (B-C) Effect of p38α MAPK siRNA transfection on induction of Nur77 expression by CCE9. HeLa229 cells transfected with p38α MAPK siRNA or control siRNA for 48 hr, and treated with 10 μM CCE9 for the indicated time were analyzed by Western blotting (B) and immunostaining (C) for Nur77 expression. (D) Effect of CCE9 on Nur77 expression and PARP cleavage in MEFs and p38α MAPK^-/-MEFs. MEFs or p38α MAPK^-/-MEFs treated with 10 μM CCE9 for 3 hr and 6 hr were analyzed by Western blotting for Nur77 protein expression and p38 MAPK activation. (E) CCE9 induced p38α MAPK-dependent Bcl-2 phosphorylation. HeLa229 cells treated for 2 hr with 10 μM CCE9 in the presence or absence of 25 μM SB203580 were subject to immunoprecipitated (IP) by using anti-Bcl-2 antibody. Immunoprecipitates were examined by Western blotting (WB) using anti-phosphorylated (P-) Ser and Thr antibodies. The same membranes were blotted with anti-Bcl-2 antibody to determine immunoprecipitation specificity. (F) Effect of SB203580 on CCE9 induced Bcl-2 conformational change. HeLa229 cells treated for 2 hr with 10 μM CCE9 in the presence or absence of 25 μM SB203580, Bcl-2 conformational change by immunostaining with the anti-Bcl-2 (BH3) antibody. (G) Effect of p38α MAPK siRNA transfection on CCE9 induced Bcl-2 conformational change. HeLa229 cells transfected with p38α MAPK siRNA or control siRNA for 48 hr, and treated with 10 μM CCE9 for 2 hr, Bcl-2 conformational change by immunostaining with the anti-Bcl-2 (BH3) antibody.