Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 15;8(41):69746–69755. doi: 10.18632/oncotarget.19261

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © 2017 Zhou et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

(A) The stable cells of BCPAP and TPC-1 cells overexpressing let-7a were transfected with AKT2 cDNA without 3’-UTR region. After 72 h cultures, immunoblotting assay was performed as described above. The density of relative proteins expression levels were quantified and normalized to the level of GAPDH. (B) The Cell Counting Kit-8 (CCK-8) assay showed that BCPAP and TPC-1 cells stably expressing let-7a grew slower than cells stably expressing miR-NC. (C, D, E) Colony formation, migration and transwell invasion assays of BCPAP and TPC-1 cells stably expressing miR-NC or let-7a were performed. Error bars represent the mean±SD of triplicate experiments. (*, **) Significant difference when compared with the miR-NC group (P< 0.05)(P< 0.01). (^#, ^##) Significant difference when compared with the let-7a group (P< 0.05, P< 0.01). Bar =200μm.

(A) The stable cells of BCPAP and TPC-1 cells overexpressing let-7a were transfected with AKT2 cDNA without 3’-UTR region. After 72 h cultures, immunoblotting assay was performed as described above. The density of relative proteins expression levels were quantified and normalized to the level of GAPDH. (B) The Cell Counting Kit-8 (CCK-8) assay showed that BCPAP and TPC-1 cells stably expressing let-7a grew slower than cells stably expressing miR-NC. (C, D, E) Colony formation, migration and transwell invasion assays of BCPAP and TPC-1 cells stably expressing miR-NC or let-7a were performed. Error bars represent the mean±SD of triplicate experiments. (*, **) Significant difference when compared with the miR-NC group (P< 0.05)(P< 0.01). (^#, ^##) Significant difference when compared with the let-7a group (P< 0.05, P< 0.01). Bar =200μm.