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. 2017 Jul 25;8(41):70006–70034. doi: 10.18632/oncotarget.19549

Figure 4. Verification the subcellular localization of some EBV representative proteins fused with Flag tag from each category (Tables 1 to 3) using IFA.

Figure 4

The plasmids expressing Flag fused BFRF3 and BMRF1 from nuclear localization, BSLF1 and BGLF1 from cytoplasmic localization, BGLF2 and BSRF1 from pan-cellular localization were transfected into COS-7 cells, and cells were subjected to IFA 24 h after transfection using anti-Flag mAb and FITC-conjugated goat anti-mouse IgG. Pictures were obtained using a Zeiss Axiovert 200M microscope. The same magnification was used in all panels. Representative fluorescence images of the vast majority live cells expressing indicated fusion protein were shown. Cells were counterstained with Hoechst to visualize the nuclear DNA. All scale bars indicate 10 μm.