Table 2. Cytoplasmic localization summary of EBV-encoded proteins.
| No. | Protein name(s) | Function/description # | COS-7 subcellular localization | Literature localization and references |
|---|---|---|---|---|
| 1 | BILF2 m | Potential membrane glycoprotein gp55/80, Ig-like, gp78 | Subcytoplasmic, in intense perinuclear, ER or Golgi like | N/A |
| 2 | BLRF1 m | Membrane glycoprotein gN, part of the gM-gN complex, part of the envelope-tegument interaction, homology with HSV-1 UL49A, VZV ORF9a, HHV5 UL73, MCMV M73 and KSHV ORF53 | Subcytoplasmic, ER like | Subcytoplasmic, ER-like a [6] |
| 3 | BXLF2 m | Glycoprotein H, gp85, part of gHgLgp42, homology with HSV-1 UL22, VZV ORF37, MCMV M75 and KSHV ORF22 | Pan-Cytoplasmic | Cytoplasm and nuclear rim a [65] |
| 4 | BLLF1 m | Envelope glycoprotein gp340/gp220, gp350, initial cell binding through complement receptor 2 (CR2, CD21) | Subcytoplasmic, perinuclear concentration, ER or Golgi like, | Golgi and plasma membrane b [20, 66] |
| 5 | BKRF2 m | Glycoprotein L, gp25, in gL-gH complex involved in viral fusion together with gB, homology with HSV-1 UL1, VZV ORF60, MCMV M115 and KSHV ORF47 | Subcytoplasmic, in intense perinuclear | N/A |
| 6 | BFLF1 p | Major envelope protein, plays role in DNA packaging, cytosolic zinc-binding protein, cysteine rich, homology with HSV-1 UL32, VZV ORF26, MCMV M52 and KSHV ORF68 | Pan-Cytoplasmic | N/A |
| 7 | BBLF2/3 r | Primase-associated factor, spliced, full sequence not in DB, part of helicase–primase complex | Pan-Cytoplasmic | Pan-Cellular, Cytoplasmic > Nuclear a [6] Cytoplasmic localization or mixed cytoplasmic plus nuclear localization a [67] |
| 8 | BNLF1 l (LMP1) |
Latent membrane protein, interferes with signalling, TRAF-binding through CTAR1 and 2, essential for immortalization | Subcytoplasmic, with speckle structures | Cytosolic, cytoskeletal, lipid raft and internal perinuclear membranes a [68] |
| 9 | LMP2A l | Latent membrane protein, interference with protein kinase signalling, gene terminal protein, essential for immortalization, homology with KSHV K15 | Subcytoplasmic, perinuclear speckle concentration | Perinuclear regions, plasma membrane a [69] |
| 10 | LMP2B l | Latent membrane protein, negative regulator of LMP-2A | Subcytoplasmic, nuclear membrane, like trans-Golgi network | Perinuclear regions and trans-Golgi network a [69, 70] |
| 11 | BCRF1 t (vIL-10) | Viral interleukin-10 homologue precursor, vIL-10, homology with MCMV M87 and KSHV ORF24 | Pan-Cytoplasmic | Subcytoplasmic a [6] |
| 12 | BRRF2 T | Unknown function, homology with KSHV ORF48 | Pan-Cytoplasmic | Subcytoplasmic a [6] |
| 13 | BSLF1 n | Primase, DNA helicase-primase component, homology with HSV-1 UL52, VZV ORF6, MCMV M70 and KSHV ORF56 | Pan-Cytoplasmic | Pan-cytoplasmic a [6] |
| 14 | BFRF2 u | Possible capsid protein, not included in virions, unknown function, homology with MCMV M49 and KSHV ORF66 | Pan-Cytoplasmic | Pan-cytoplasmic a [6] |
| 15 | BBRF1 c | Minor capsid protein portal protein UL6 homologue, homology with HSV-1 UL6, VZV ORF54, MCMV M104 and KSHV ORF43 | Subcytoplasmic, with speckles in the cytoplasmic | Pan-cytoplasmic a [6] |
| 16 | BMRF2 m | Receptor for cellular integrins, needed for infection of epithelial cells, 10 TM helices, RGD motif, membrane accociated protein, homology with KSHV ORF58 | Subcytoplasmic, cytoplasmic obviously > Nuclear, perinuclear concentration, ER or Golgi like | Cytoplasm and membrane a/b [71] ER and Golgi a [72] |
| 17 | BALF1 t | Bcl-2 homologue, negative regulator of anti-apoptosis protein BHRF1 | Pan-Cytoplasmic | Cytoplasmic > Nuclear a [6] Cytoplasmic a [73] |
| 18 | ECRF4 u | Unkown function | Cytoplasmic obviously > Nuclear | N/A |
| 19 | BALF4 m | Glycoprotein B, glycoprotein 110, fusion and co-receptor binding, homology with HSV-1 UL27 (gB), VZV ORF31, MCMV M55 and KSHV ORF8 | Cytoplasmic obviously > Nuclear, intense in perinuclear cytoplasmic with some patches, ER or Golgi like | Cytoplasmic, perinuclear and nuclear membranes a [74] Cytoplasmic, nuclear membranes, ER and the inner/outer nuclear membranes, plasma membrane and cell surface b [66, 75] |
| 20 | BILF1 m | G-protein coupled receptor (G-PCR), includes 7 transmembrane helices, 6 glycosylation sites, 2 disulfide bridges gP64 | Pan-Cytoplasmic, plasma membrane like | Plasma membrane a [76, 77] Cytoplasmic membrane b [78] |
| 21 | BARF1 t | CD80 homologue (p33), oncogene, soluble glycoprotein, includes 2 Ig domains, binds CSF-1, homology with HSV-1 UL40, VZV ORF18 and KSHV ORF60 | Cytoplasmic obviously > Nuclear | Cytoplasmic membrane a/b [79] Subcytoplasmic, ER-like, perinuclear concentration a [6] |
| 22 | BVLF1 u | Unknown function | Cytoplasmic obviously > Nuclear | N/A |
| 23 | LF1 u | Contains a dUTPase like domain, γ-herpes ORF10 family | Cytoplasmic obviously > Nuclear | N/A |
| 24 | BNLF2a u | Potential membrane protein | Cytoplasmic obviously > Nuclear, ER or Golgi like | Pan-Cytoplasmic a [6] ER a [80] |
| 25 | BGLF1 T | Potential tegument protein (gp115), homology with HSV-1 UL17, VZV ORF43, MCMV M93 and KSHV ORF32 | Pan-Cytoplasmic | Pan-Cytoplasmic a [6] |
| 26 | BORF2 n | Ribonucleotide-reductase, large subunit, 140 kDa, homology with HSV-1 UL39, VZV ORF19, MCMV M45 and KSHV ORF61 | Cytoplasmic obviously > Nuclear, with punctates in the cytoplasmic | Subcytoplasmic, punctate cytoplasmic crystal-like structures a [6] |
| 27 | BXLF1 n | Thymidine kinase, homology with HSV-1 UL23, VZV ORF36 and KSHV ORF21 | Subcytoplasmic, irregularly shaped cytoplasmic structures | Subcytoplasmic, irregularly shaped cytoplasmic structures a [6] |
| 28 | BBLF4 r | Helicase, part of helicase–primase complex, homology with HSV-1 UL5, VZV ORF55, MCMV M105 and KSHV ORF44 | Cytoplasmic obviously > Nuclear | Cytoplasmic > Nuclear a [81] |
| 29 | BALF5 r | DNA polymerase, homology with HSV-1 UL30, VZV ORF28, MCMV M54 and KSHV ORF9 | Pan-Cytoplasmic | Nuclear b [21] |
| 30 | BTRF1 c | Hypothetical protein, involved in capsid maturation, capsid-associated, homology with HSV-1 UL21, VZV ORF38, MCMV M88 and KSHV ORF23 | Pan-Cytoplasmic | Nuclear > Cytoplasmic a [6] |
| 31 | LF2 u | Contains a dUTPase like domain, γ-herpes ORF11 family | Cytoplasmic, cytoskeletal like | Cytoplasmic, cytoskeletal a [82] |
| 32 | BDLF2 T | Potential type II glycosylated envelope protein, homology with KSHV ORF27 | Subcytoplasmic, with perinuclear concentration | Subcytoplasmic, ER-like a [6, 72] |
a: transfection
b: infection
N/A: Not Detected
r: replication related protein
n: nucleotide metabolism related protein
t: transcription related protein
p: packaging related protein
l: latency related protein
m: membrane (or glycosylated) protein
c: capsid related protein
T: tegument protein
u: unkown function protein
# Function/Description column compiled the following references: Tarbouriech et al. [5] and Salsman et al. [6].