Table 3. Pan-cellular localization summary of EBV-encoded proteins.

No.	Protein name(s)	Function/description #	COS-7 subcellular localization	Literature localization and references
1	BDLF1 c	Minor capsid protein, homology with HSV-1 UL18 (VP23), VZV ORF41, MCMV M85 and KSHV ORF26	Pan-Cellular, without nucleolus	Pan-Cellular a [6, 48]
2	BBRF3 m	Integral membrane protein glycoprotein M, part of gN-gM complex involved in envelope-tegument interaction, homology with HSV-1 UL10, VZV ORF50, MCMV M100 and KSHV ORF39	Pan-Cellular	N/A
3	BDLF3 m	Membrane glycoprotein (gp150), gp117, homology with KSHV ORF28	Pan-cellular, with punctate perinuclear and cytoplasmic	Subcytoplasmic, punctate perinuclear concentration a [6]. Cytoplasmic b [83]
4	BZLF2 m	gp42, MHC class II binding protein, part of gHgLgp42 complex	Plasma membrane	N/A
5	BGRF1/BDRF1 p	DNA-packaging protein, terminase small subunit, homology with HSV-1 UL15, VZV ORF42, MCMV M89 and KSHV ORF29a	Pan-Cellular	N/A
6	BWRF1 l	EBNA-Lp (EBNA-5) nuclear phosphoprotein, highly spliced, 12 exons, proline-rich, enhances EBNA-2 transactivation	Pan-Cellular, with speckles in cytoplasm and perinuclear	N/A
7	BaRF1 n	Ribonucleotide reductase, small 38 kDa subunit, homology with HSV-1 UL40, VZV ORF18 and KSHV ORF60	Pan-Cellular	Pan-Cellular a [6]
8	BKRF3 n	Uracil DNA glycosylase, homology with HSV-1 UL2, VZV ORF59, MCMV M114 and KSHV ORF46	Pan-Cellular	Pan-Cellular a [6, 84] Translocated from the cytoplasm into the nucleus b [21]
9	BFRF4 p	DNA-cleavage and packaging protein, part of the DNA packaging machinery (BFRF0.5, HS4BAM), homology with HSV-1 UL33, VZV ORF25, MCMV M51 and KSHV ORF67.5	Pan-Cellular	N/A
10	BHRF1 t	Early antigen protein R (EA-R), nuclear antigen, anti-apoptotic factor Bcl-2 homologue	Pan-Cellular, with speckles in cytoplasm and perinuclear, mitochondria like	Mitochondria, ER and nuclear membranes a [85] Mitochondria b [86]
11	LF3 u	Unknown function	Pan-Cellular	N/A
12	BSLF2 u	N-terminal fragment (5' exon) of SM/EB2 protein and part of BSLF2/BMLF1 protein, homology with HSV-1 UL54, VZV ORF4, MCMV M69 and KSHV ORF57	Pan-Cellular, without nucleolus	Pan-Cellular a [6, 48]
13	BGLF3.5 u	Unknown function, homology with MCMV M96 and KSHV ORF35	Pan-Cellular, without nucleolus	N/A
14	BFRF1A u	Unknown function	Pan-Cellular, without nucleolus	N/A
15	BNLF2b u	Potential gp141	Pan-Cellular, cell membrane	N/A
16	BcRF1 u (UL87)	Unknown function, herpesvirus UL87 family, homology with MCMV M87 and KSHV ORF24	Pan-cellular, with speckle cytoplasmic structures	Subcytoplasmic a [6]
17	BBRF2 T	Unknown function, homology with HSV-1 UL7, VZV ORF53, MCMV M103 and KSHV ORF42	Pan-Cellular	Pan-Cellular a [6]
18	BFRF1 m	Nuclear membrane protein p38, transmembrane with large cytoplasm domain, complex with BFLF2, homology with HSV-1 UL34, VZV ORF24, MCMV M50 and KSHV ORF67	Subcytoplasmic and perinuclear region, with speckle cytoplasmic structures	Pan-Cytoplasmic and perinuclear region a [6, 87] Nuclear envelope b [88]
19	BGLF2 T	MyrPBP, homology with HSV-1 UL16, VZV ORF44, MCMV M94 and KSHV ORF33	Pan-Cellular, without nucleolus	Pan-Cellular a [6] Nuclei with granular b [89]
20	BSRF1 T	Palmitoylated tegument protein (PalmP), homology with HSV-1 UL51, VZV ORF7, MCMV M71 and KSHV ORF55	Pan-Cellular	Subcytoplasmic, perinuclear concentration a [6]
21	BVRF1 T	Capsid associated protein, portal plug (EC-RF2), seals DNA inside capsid, homology with HSV-1 UL25, VZV ORF34, MCMV M77 and KSHV ORF19	Pan-Cellular	Pan-Cellular, Cytoplasmic > Nuclear a [6]

a: transfection

b: infection

N/A: Not Detected

r: replication related protein

n: nucleotide metabolism related protein

t: transcription related protein

p: packaging related protein

l: latency related protein

m: membrane (or glycosylated) protein

c: capsid related protein

T: tegument protein

u: unkown function protein

^# Function/Description column compiled the following references: Tarbouriech et al. [5] and Salsman et al. [6].