Figure 6. Microscopic evaluation of mammary gland tissue of the animal treated with ALA.
Whole mount carmine alum staining of ductal epithelium reveals the presence of lobules (1) and AB (2) (A, B, C and D). The extent of AB and lobules formation was excessive in the DMBA treated group (B) which was subsided through respective treatment (C and D). H&E staining of three respective groups (E, G and H) revealed duct (3), adipocytes (4), LCT (5), DCT (6), MEC (7), lymphocytes (8) and CEC (9) in control as well as ALA treated groups (E, G and H). In DMBA treated group (F), the cell morphology was distorted and cell organelles were absent. SEM analysis of control (I), DMBA treated (J) and treatment group (K and L) revealed the differences in collagen layer (10), duct (11), small capillary network (12) and nodules (13) in respective groups.