Figure 3. Deregulated expression of PIAS4 or SENP2 results in abnormal mitotic cycle.
(A and B) U2OS cells stably expressing PIAS4 shRNA (A) or SENP2 (B) were stained with DAPI. Nuclear morphology was shown. Scale bar: 10uM. (C) Duplication of chromosome. DNA FISH using a chromosome 19 satellite repeat probe (red) in cells stably expressing PIAS4 shRNA. Scale bar: 10uM. (D) Typical image of metaphase chromosome spread of cells stably expressing SENP2. (E and F) 2X105 PIAS4-knocking down cells or SENP2-overexpressing cells or control cells were plated and cell number was counted every day for five days. The graph shows the average of three independent experiments; mean ± S.D.; *, p < 0.05; **, p < 0.01. (G and H) Cell cycle profiles of PIAS4 shRNA-expressing cells (G) and SENP2-overexpressing cells (H) are shown.