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. 2017 Jul 26;8(41):70142–70155. doi: 10.18632/oncotarget.19594

Figure 4. Deregulated expression of PIAS4 or SENP2 results in by pass of mitosis.

Figure 4

(A and B) U2OS cells stably expressing PIAS4 shRNA (A) or SENP2 (B) were treated with or without nocodazole for 20 hours. Cell image (upper panels) and Mitotic index (lower panels) was shown. (C and D) U2OS cells stably expressing PIAS4 shRNA (C) or SENP2 (D) were treated with or without nocodazole for 20 hours. Western blot was performed to detect expressions of different proteins as indicated. (E and F) U2OS cells stably expressing PIAS4 shRNA (E) or SENP2 (F) were treated with mimosine for 24 hours. Cells were released into fresh medium for different times. Western blot was performed to detect expression of phosphorylated histone H3 Ser 10. (G and H) U2OS cells stably expressing PIAS4 shRNA (G) or SENP2 (H) were treated with or without nocodazole for 24 hours. Cell cycle profiles were analyzed by FACS.