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. 2017 Jul 26;8(41):70192–70203. doi: 10.18632/oncotarget.19599

Figure 4. Targeting the Gly-Leu-Ser/Lys- “Co-adaptation pocket,” PTL suppresses EMT via ERK 2/NF-κB/Snail pathway.

Figure 4

(A and C) Gene expression microarray was used to interrogate the differentially expressed genes. (B) A total of 164 influenced pathways of the different expression proteins predicted from different expression genes, with which 137 of 140 were similar to the reversely predicted based PTL activity. (D) POVME 2.0 algorithm was used to measure and characterize the active pocket of proteins. (E)“Co-adaptation pocket” constitutes the Gly-Leu-Ser/Lys in the potential targets of PTL. (F) ERK 2 signaling was inhibited resulting in the suppression of EMT in NCI-H446. (G) Dualluciferase assay results suggest that PTL reduced NF-κB and Snail expression in NCI-H446 cell line. (H) Multiple potential targets of PTL have a “Co-adaptation pocket” and PTL inhibits ERK 2 resulting in EMT inhibition.