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. 2017 Jul 29;8(41):70317–70331. doi: 10.18632/oncotarget.19688

Figure 3. BiVax/IL2Cx but not BiVax alone or TriVax induces diabetes in Rip-gp mice.

Figure 3

(A-E) RIP-gp mice were vaccinated as described in Figure 1B. (A) Blood glucose levels in individual mice (each symbol) from at least 3 independent experiments. (B) Insulin staining in formalin fixed pancreatic tissues of WT and RIP-gp mice after TriVax or BiVax/IL2Cx boost was analyzed 8 days after the booster vaccination. (C) RIP-gp mice were primed with BiVax followed by BiVax/IL2Cx. Some mice received 500 μg of αCD8 mAb (i.p.) at days 12 and 14. Blood glucose levels were measured to assess diabetes. (D) Mean fluorescence intensity (MFI) of tetramer stains for Kb and Db specific cells in RIP-gp mice. Each symbol represents an individual mouse. (E) Purified CD8 T cells were incubated with serial dilutions of the minimal Db (KAVYNFATM) or Kb (AVYNFATM) peptides and 48 h later the production of IFNγ in the supernatants was assessed by ELISA. Dashed horizontal lines in A and C represents maximal normal blood glucose level. (*p<0.05, ns: not significant).