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. 2017 Jul 31;8(41):70550–70563. doi: 10.18632/oncotarget.19742

Figure 1. MiR-377 targets the SIRT1 3′-UTR and downregulates SIRT1 protein abundance at the translational level.

Figure 1

(A) Relative luciferase activity of the SIRT1 3′-UTR in BHK cells after co-transfection with negative control (NC) or 18 selected potential miRNAs. *P < 0.05, **P < 0.01 vs. NC (n = 5). (B) The potential target sites were predicted by MICRORNA (http: //www. Microrna org/microrna/home.do). (C) Relative luciferase activity of wild-type or mutant 3′-UTR after co-transfection with miR-377, miR-132 or miR-146b mimics. *P < 0.05, **P < 0.01 vs. NC; ##P < 0.01 vs. miR-377 SIRT1 3’UTR WT; ns, not significant (n = 5). (D–F) Mature 3T3-L1 cells were transfected with 100 nM miR-377 mimics for 24 h and then harvested for real-time PCR and immunoblotting analyses. **P < 0.01 vs. NC (n = 3). (D) MiR-377 expression. (E) SIRT1 mRNA levels. (F) SIRT1 protein levels.