Distribution of temporal classes of gap gene expression patterns in timed egg collections. (A) Early gap gene (hb, Kr, and mlpt) expression patterns are each classified into three temporally consecutive classes: class Φ for early weak maternal expression (hb) or no expression (Kr and mlpt), class I for high expression at the posterior, and class II for an expression turning off at posterior. (B) Percentages of embryos belonging to each class are calculated for consecutive 3-h developmental windows covering the blastoderm and early germband stages of Tribolium: 14 h to 17 h, 17 h to 20 h, 20 h to 23 h, and 23 h to 26 h AEL.