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. 2017 Sep 25;114(41):10918–10923. doi: 10.1073/pnas.1704030114

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Fig. 5. — Cytoplasmic YAP regulates CDC42 activity in HUVEC. (A) Scratch assay of YAP/TAZ knockdown HUVECs expressing Venus or YAPS127D. (B) Quantification of invaded area within the scratched region (n = 3); mean ± SD, *P < 0.01, N.S., not significant; S127D, YAPS127D; Ven, venus. (C) Scratch assay of YAP/TAZ knockdown HUVECs expressing YAPS112A. (D) Quantification of invaded area within the scratched region (n = 3); mean ± SD, N.S., not significant; S112A, YAPS112A. (E) Active-CDC42 pull-down assay of YAPS127D lentivirus-infected (S127D) and YAPS112A adenovirus-infected (S112A) HUVECs. CDC42, YAP, and GAPDH expression levels are detected by Western blot analysis. [Scale bar: 50 μm (A and C).]