Fig. S5.

Isolation of brain VECs from neonatal mice. Brain VECs were isolated from P5 neonates as described in SI Materials and Methods. Quantitative PCR analysis for Tek/Tie2 expression indicated efficient separation of VECs and non-VECs; mean ± SD, *P < 0.01. The Yap and Taz mRNA expression was significantly down-regulated in Yap/Taz cKO brain VECs. The expression of YAP target genes (Ctgf and Cyr61) was down-regulated in Yap/Taz cKO and was up-regulated in Lats1/2 cKO brain VECs; mean ± SD, *P < 0.01.