Fig. S6.

Correlation between cytoplasmic YAP (phospho-YAP) and CDC42 activity in HUVECs. (A) Endogenous YAP localization in 70% or overconfluent HUVECs. (B) Phospho-YAP (P-YAP) and GTP-bound CDC42 (active-CDC42) expression in different cell densities are determined by immunoblotting. Active-CDC42 pull-down assay was performed as described in Materials and Methods. (C) Endogenous YAP localization in migrating HUVECs. Overconfluent HUVECs are scratched, incubated for 0, 4, or 8 h, and then stained with YAP antibody (green) and phalloidin (red). (D and E) Western blot analysis for phosphorylated-YAP and active-CDC42 pull-down assay using LATS1/2 knockdown HUVECs and brain endothelial cells isolated from P5 Lats1/2 cKO neonates.