Table S2.

Gel filtration elution volume (Ve) for various gHgL/gp42 complexes with anti-gHgL Fab

Sample no.	Glycoprotein complex	Ve, mL	Apparent molecular mass, kDa*
1	CL40 Fab/gHgL	11.61	179.4
2	CL59 Fab/gHgL	11.73	169.9
3	E1D1 Fab/gHgL	11.52	186.8
4	CL40 Fab/gHgL/gp42(47–81)†	11.62	178.6
5	CL59 Fab/gHgL/gp42(47–81)	11.52	186.8
6	E1D1 Fab/gHgL/gp42(47–81)	11.39	198.1
7	CL40 Fab/gHgL/gp42 WT	11.23	212.9
8	CL59 Fab/gHgL/gp42 WT	11.05	230.9
9	E1D1 Fab/gHgL/gp42 WT	10.60	282.8
10	E1D1 Fab/CL40 Fab/gHgL/gp42(47–81)	10.80	258.4
11	CL59 Fab/E1D1 Fab/CL40 Fab/gHgL/gp42(47–81)	10.41	308.1

^*Apparent molecular mass was calculated using the following formula after S200 calibration: log₁₀(molecular mass, kDa) = −0.1958 × (Ve, mL) + 4.527; R² = 0.9912; column efficiency: N/m = 12,137 (acceptable, N/m >10,000); peak symmetry: As = 1.227 (acceptable, 0.70 < As < 1.30).

^†gp42(47–81) or gp42 35merA1¹⁹ sequence: ⁴⁷KPNVEVWPVDPPPPVNFNKTAEQEYGDKEVKLPHW⁸¹.