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. 2017 Sep 22;114(41):E8703–E8710. doi: 10.1073/pnas.1704661114

Table 2.

SPR steady-state analysis to derive KD

Stationary phase (ligand) Surface density of ligand, RU* Mobile phase (analyte) Rmax KD, nM
HLA-DQ2 (α1) 750 gHgL/gp42 79.6 134.6
HLA-DQ2 (α1) 750 CL40/gHgL/gp42 63.7 45.1
*

Ligand density rounded off to the nearest tenth after subtracting RU value of baseline level after a deactivation step with ethanolamine for the ligand channel minus the corresponding reference channel (Ch4-Ch1 or Ch3-Ch2).