Table 2.
Stationary phase (ligand) | Surface density of ligand, RU* | Mobile phase (analyte) | Rmax | KD, nM |
HLA-DQ2 (α1) | 750 | gHgL/gp42 | 79.6 | 134.6 |
HLA-DQ2 (α1) | 750 | CL40/gHgL/gp42 | 63.7 | 45.1 |
Ligand density rounded off to the nearest tenth after subtracting RU value of baseline level after a deactivation step with ethanolamine for the ligand channel minus the corresponding reference channel (Ch4-Ch1 or Ch3-Ch2).